Evidence for Molecular Heterogeneity of the Specific Antigen (Fraction-1) of Pasteurella pestis.
نویسندگان
چکیده
Fraction-I (F-I) protein, the specific antigen of Pasteurella pestis, appears to be a series of molecular aggregates of identical subunits, all of which have serological reactivity with specific F-I antiserum.
منابع مشابه
The Identification and Isolation of Two Mouse-toxic Protein Components in Extracts from Pasteurella Pestis
The toxin activity of Pasteurella pestis cells, strain "Tjiwidej," was found to be associated with two proteins. Using a disc electrophoresis technique in conjunction with mouse lethality, two toxic proteins were isolated exhibiting intraperitoneal LD(50)'s of less than 1.0 to 1.5 microg protein. Each produced a single characteristic precipitin band on agar gel diffusion plates. The slower migr...
متن کاملPesticinogeny: a characteristic useful for presumptive identification and isolation of Pasteurella pestis.
Current methods of identifying Pasteurella pestis rely heavily on tests specific for detecting fraction I, the envelope antigen. Pesticin I, a bacteriocin inhibitory for P. pseudotuberculosis, has been demonstrated in nearly all tested strains isolated from human infections. The results of using this characteristic as an identifying trait for P. pestis were compared with results reported for de...
متن کاملProduction of Pasteurella pestis toxin.
In the course of the development of a casein hydrolyzate mineral glucose medium for the growth of Pasteurella pestis, strain A1122, for the production of the envelope antigen (Englesberg and Levy, 1954), the potential use of this medium for plague toxin production became apparent. The development of a simplified method for the production of plague toxin made possible by this medium will be desc...
متن کاملMolecular Typing of Avian Pasteurella multocida lsolatesby PCR-RFLPof ompH Gene
Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragmentlength polymorphism (RFLP) of a species-specific PCR assay. Amplification was based on the gene ompH, encoding a major outer memberane protein. RFLP analysis of the 1.2 kb ompH-amplification usingEcoRI, HindIII and CfoI endonucleases produced 7 different patterns for the twenty fi...
متن کاملIsolation of Antigens of Pasteurella pestis I. Lipopolysaccharide-Protein Complex and R and S Antigens.
Larrabee, Allan R. (Fort Detrick, Frederick, Md.), John D. Marshall, and Dan Crozier. Isolation of antigens of Pasteurella pestis. I. Lipopolysaccharide-protein complex and R and S antigens. J. Bacteriol. 90:116-119. 1965.-Pasteurella pestis contains at least 18 different antigens, 2 of which will protect experimental animals from challenge infection. A specific polysaccharide isolated and desc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Infection and immunity
دوره 3 3 شماره
صفحات -
تاریخ انتشار 1971